Dr Hooi Yuan Teng

Research Fellow

Jeffrey Cheah School of Medicine & Health Sciences

Email: YuanTeng.Hooi@monash.edu

ORCID ID: https://orcid.org/0000-0002-3690-6969

Profile Summary

Dr. Hooi Yuan Teng graduated from University of Malaya, majoring in Bioinformatics in 2011. After few years of working as a research assistant with Professor Dr. Wong Kum Thong, a neuropathologist in the Department of Pathology, University of Malaya. She then pursued her doctoral degree under his supervision. Her PhD research project was to develop an animal model for Coxsackievirus A16 (CV-A16) infection that causes hand-foot-mouth disease (HFMD), and to understand the infectious pathology and pathogenesis studies of CV-A16. She graduated in 2020 and currently works as a postdoctoral research fellow under Dr. Vinod RMT Balasubramaniam in the laboratory of the Infection and Immunity Cluster, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia.

She has experience in animal works specializing in histopathological techniques (immunohistochemistry, in situ hybridization and etc.) and virus handling (virus culture, viral titration, PCR, qRT-PCR and etc.). In the past years, she had published several peer-reviewed journal articles, including 1 from the list of Tier 1 journals (Laboratory Investigation). She had also presented in international conferences and awarded with “Best Poster Prize” in one of the conferences (Hand, Foot and Mouth Disease International Conference 2016, Biopolis, Singapore).

Work Experience

  • Research Fellow, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia (February 2021 – present)
  • LOCUM Clinical Lab Scientist, Lablink Medical Laboratory, Kuala Lumpur (March – June 2020)
  • Research Assistant, Department of Biomedical Science, Faculty of Medicine, University of Malaya (June 2018 – November 2019)
  • Research Assistant, Department of Pathology, Faculty of Medicine, University of Malaya (June 2011 – August 2014)
  • Non-Trained Substitute Teacher at SRJK(C) Kwong Hon (January – June 2008)

Qualifications

  • 2014 – 2020 Doctor of Philosophy, University of Malaya
  • 2011 Summer Program for Laboratory Research, National Taiwan University
  • 2008 – 2011 Bachelor of Science (Bioinformatics), University of Malaya

Research / Education interests

Enteroviruses are associated with several human and mammalian diseases. Both Coxsackievirus A16 (CV-A16) and Enterovirus A71 (EV-A71) from Enterovirus A species could cause hand-foot-mouth disease (HFMD) in humans, which usually presents as a mild febrile exanthematous disease with vesicles/rashes on the palmar and plantar skin, and ulcers on the buccal mucosa and tongue. In contrast to EV-A71 encephalomyelitis, little is known about the central nervous system pathology in CV-A16 infection because to date there is no human autopsy study available on this important CV-A16 complication. In Dr. Hooi’s previous research project, she had developed CV-A16 animal models for pathogenesis and infectious disease pathology studies. The findings not only confirmed CV-A16 neurotropism, and suggested skeletal muscle and brown adipose tissue as important viral replication sites. The kinetic studies suggested neuroinvasion is possible via retrograde axonal transport in peripheral motor nerves.

Some of Dr. Hooi’s previous work include:

  • Development of specific CV-A16 and EV-A71 DNA probes for in situ hybridization (ISH) in infected formalin-fixed paraffin embedded tissues.
  • Comparison of infectious disease pathology of CV-A16 and EV-A71 in mouse model.
  • Development of an orally-infected CV-A16 HFMD hamster model for the study of viral distribution and tissue tropism, viral replication kinetics, viral shedding and transmission among host animals.
  • Phenotypic and genotypic characterization of mouse adapted CV-A16 strains.

Research Interest

Virology, Infectious disease, Pathogenesis, Animal model, Histopathology

Research Strengths

Virology, cell and tissue culture, animal handling, microtomy, immunohistochemistry, in situ hybridization, molecular biology

Publications

Journals

Hooi, Y. T., Ong, K. C., Perera, D., & Wong, K. T. (2015). Pathological findings in a mouse model for Coxsackievirus A16 infection. Neurology Asia, 20(4), 343-347.

Hooi, Y. T., Ong, K. C., Tan, S. H., Perera, D., & Wong, K. T. (2020). Coxsackievirus A16 infection in a day-old mouse model of CNS infection shows lower neurovirulence than Enterovirus A71. Journal of Comparative Pathology, 176, 19-32.

Hooi, Y. T., Ong, K. C., Tan, S. H., Perera, D., & Wong, K. T. (2020). A novel orally-infected hamster model for Coxsackievirus A16 hand-foot-and-mouth disease and encephalomyelitis. Laboratory Investigation, 100(9), 1262-1275.

Conferences

The Second International Conference on Tropical Medicine & Infectious Diseases. Diseases Without Borders: Challenges and Preparedness. 29th September – 1st October 2015, Ipoh, Malaysia. Hooi, Y. T., Ong, K. C., & Wong, K. T. Development of a Digoxigenin-Labeled DNA Probe for the Detection of Coxsackievirus A16 RNA in Tissues using In Situ Hybridisation. 

Hand, Foot and Mouth Disease International Conference 2016. 25th – 26th July 2016, Biopolis, Singapore. Hooi, Y. T., Ong, K. C., Perera, D. & Wong, K. T. Pathological findings in a mouse model for Coxsackievirus A16 infection. 

17th Asian and Oceanian Myology Centre (AOMC) Annual Scientific Meeting and 28th Malaysian Society of Neurosciences (MSN) Annual Scientific Meeting. Synapses and Beyond. 28th – 29th July 2018, Kuala Lumpur, Malaysia. Hooi, Y. T., Ong, K. C., Perera, D. & Wong, K. T. Coxsackievirus A16 and Enterovirus A71 infections in a one-day old mouse model show similar neuronal involvement in brainstem and spinal cord.

The 19th International Congress of Neuropathology. A Gateway to Modern Neuroscience. 23rd – 27th September 2018, Tokyo, Japan. Hooi, Y. T., Ong, K. C., Perera, D. & Wong, K. T. An orally-infected hamster model for Coxsackievirus A16 infection confirms neurovirulence.

Research Grants

Postgraduate research grant by University of Malaya (Jan 2016)
Position: Co-researcher
Title: Coxsackievirus A16 and Enterovirus 71 infection: Pathogenetic studies using mouse models.
Hooi Yuan Teng, Professor Dr. Wong Kum Thong

Research Supervision

Awards and Achievements

National
Awarded Scholarship (Skim Biasiswazah) by Institute of Graduate Studies, University of Malaya

International
Awarded Best Poster Prize in Hand, Foot and Mouth Disease International Conference 2016
Awarded Travel Grant for Hand, Foot and Mouth Disease International Conference 2016